Inhibitory Effect of Gallic Acid and Its Esters on 2,2 '-Azobis(2-amidinopropane)hydrochloride (AAPH)-Induced Hemolysis and Depletion of Intracellular Glutathione in Erythrocytes


Autoria(s): Ximenes, Valdecir F.; Lopes, Mariana G.; Petronio, Maicon Segalla; Regasini, Luis Octavio; Siqueira Silva, Dulce H.; da Fonseca, Luis M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

12/05/2010

Resumo

The protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on proapoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.

Formato

5355-5362

Identificador

http://dx.doi.org/10.1021/jf100233y

Journal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 58, n. 9, p. 5355-5362, 2010.

0021-8561

http://hdl.handle.net/11449/7286

10.1021/jf100233y

WOS:000277237500021

Idioma(s)

eng

Publicador

Amer Chemical Soc

Relação

Journal of Agricultural and Food Chemistry

Direitos

closedAccess

Palavras-Chave #Gallic acid #gallates #erythrocytes #hemolysis #glutathione #peroxyl radical #antioxidant #pro-oxidant activity
Tipo

info:eu-repo/semantics/article