Botryosphaeran, a new substrate for the production of beta-1,3-glucanases by Botryosphaeria rhodina and Trichoderma harzianum Rifai


Autoria(s): Giese, E. C.; Covizzi, L. G.; Borsato, D.; Dekker, RFH; da Silva, M. D.; Barbosa, A. M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/12/2005

Resumo

Botryosphaeria rhodina and Trichoderma harzianum Rifai were grown on botryosphaeran (an exopolysaccharide (EPS) of the beta-1,3; 1,6-D-Glucan type produced by B. rhodina) as sole carbon source with the objective of producing beta-glucanases of the beta-type. Conditions for beta-1,3-glucanase production by T harzianum were examined by a statistical response surface method, and showed maximal enzyme production at 5 days growth in media containing 1.5 g/1 of EPS. Good agreement was obtained between the experimental values of beta-1, 3-glucanase activity and the corresponding values predicted by the mathernatical model. The crude beta-1,3-glucanase preparations were active towards a number of different beta-1,3-glucans and beta-glucosides. The mycelium of B. rhodina also proved to be a good substrate for beta-1,3-glucanase production by both fungal species. (c) 2005 Published by Elsevier Ltd.

Formato

3783-3788

Identificador

http://dx.doi.org/10.1016/j.procbio.2005.04.004

Process Biochemistry. Oxford: Elsevier B.V., v. 40, n. 12, p. 3783-3788, 2005.

1359-5113

http://hdl.handle.net/11449/6812

10.1016/j.procbio.2005.04.004

WOS:000233068200019

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Process Biochemistry

Direitos

closedAccess

Palavras-Chave #Botryosphaeran #Botryosphaeria rhodina #exopolysaccharide beta-1,3-glucanases #Trichoderma harzianus Rifai #response surface methodology
Tipo

info:eu-repo/semantics/article