Effects of Aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages


Autoria(s): Facchini, Fernanda D. A.; Reis, Victor R. A.; Roth, Anna Paula; Magalhaes, Karla A.; Peixoto-Nogueira, Simone C.; Casagrande, Daniel R.; Reis, Ricardo A.; Polizeli, Maria de Lourdes T. M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/09/2012

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Processo CNPq: 563260/2010-6/FAPESP

BACKGROUND: Cellulose and hemicellulose are quantitatively the most important structural carbohydrates present in ruminant diets. Rumen micro-organisms produce enzymes that catalyse their hydrolysis, but the complex network formed by structural carbohydrates and lignin reduces their digestibility and restricts efficient utilisation of feeds by ruminants. This study aimed to produce two enzymatic extracts, apply them in ruminant diets to determine the best levels for ruminal digestibility and evaluate their effects on in vitro digestibility. RESULTS: In experiment 1 a two-stage in vitro technique was used to examine the effects of different enzymatic levels of Aspergillus japonicus and Aspergillus terricola on tropical forages. Enzyme addition had minor effects on corn silage at the highest enzymatic level. In experiment 2 an in vitro gas production (GP) technique was applied to determine apparent in vitro organic matter digestibility and metabolisable energy. The addition of enzymes in GP showed interesting results. Good data were obtained using sugar cane and Tifton-85 hay supplemented with extracts of A. japonicus and A. terricola respectively. CONCLUSION: Overall, the study suggests that addition of crude extracts containing exogenous fibrolytic enzymes to ruminant diets enhances the effective utilisation of ruminant feedstuffs such as forages. Copyright (c) 2012 Society of Chemical Industry

Formato

2569-2573

Identificador

http://dx.doi.org/10.1002/jsfa.5684

Journal of The Science of Food and Agriculture. Hoboken: Wiley-blackwell, v. 92, n. 12, p. 2569-2573, 2012.

0022-5142

http://hdl.handle.net/11449/4648

10.1002/jsfa.5684

WOS:000307164500023

Idioma(s)

eng

Publicador

Wiley-Blackwell

Relação

Journal of the Science of Food and Agriculture

Direitos

closedAccess

Palavras-Chave #Aspergillus spp #fibrolytic enzymes #gas production technique #in vitro digestibility #tropical forages
Tipo

info:eu-repo/semantics/article