Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)


Autoria(s): Silva, Paula Viana Correa da; Lui, Jeffrey Frederico; Band, Guilherme de Oliveira; Regitano, Luciana Correia de Almeida; Grossi, Selma de Fátima; Sollero, Bruna Pena; Nunes, Cleujosí da Silva
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/04/2011

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.

Formato

301-306

Identificador

http://dx.doi.org/10.1590/S1516-89132011000200011

Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 2, p. 301-306, 2011.

1516-8913

http://hdl.handle.net/11449/4411

10.1590/S1516-89132011000200011

S1516-89132011000200011

WOS:000289856600011

S1516-89132011000200011.pdf

Idioma(s)

eng

Publicador

Brazilian Archives of Biology and Technology

Relação

Brazilian Archives of Biology and Technology

Direitos

openAccess

Palavras-Chave #Molecular genetics #wild boars #Microsatellites #fragments #amplification
Tipo

info:eu-repo/semantics/article