Restriction fragment length polymorphism (RFLP) in Exon 2 of the BoLA-DRB3 gene in South American cattle
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/10/2001
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Resumo |
The Bola-DRB3 gene participates in the development of the immune response and is highly polymorphic. For these reasons, it has been a candidate gene in studies of the genetic basis of disease resistance and in population genetic analysis. South American native cattle breeds have been widely replaced by improved exotic breeds leading to a loss of genetic resources. In particular South American native breeds have high levels of fertility and disease resistance. This work describes genetic variability in the BoLA-DRB3 gene in native (Caracu, Pantaneiro, Argentinean Creole) and exotic (Holstein, Jersey, Nelore, Gir) cattle breeds in Brazil and Argentina. PCR-RFLP alleles were identified by combining the restriction patterns for the BoLA-DRB3.2 locus obtained with RsaI, BstY, and HaeIII restriction enzymes. Allelic frequencies and deviations from the Hardy-Weinberg equilibrium were also calculated. Analysis of the 24 BoLA-DRB3 PCR-RFLP alleles identified showed differences in the allele distributions among breeds. |
Formato |
311-324 |
Identificador |
http://dx.doi.org/10.1023/A:1012204829894 Biochemical Genetics. New York: Kluwer Academic/plenum Publ, v. 39, n. 9-10, p. 311-324, 2001. 0006-2928 http://hdl.handle.net/11449/3939 10.1023/A:1012204829894 WOS:000172140300003 |
Idioma(s) |
eng |
Publicador |
Kluwer Academic/plenum Publ |
Relação |
Biochemical Genetics |
Direitos |
closedAccess |
Palavras-Chave | #MHC #DRB3 #Bovine #genetic variability |
Tipo |
info:eu-repo/semantics/article |