Virulence properties and antimicrobial susceptibility of Shiga toxin-producing Escherichia coli strains isolated from healthy cattle from Parana State, Brazil


Autoria(s): Pigatto, Caroline P.; Schocken-Iturrino, Ruben P.; Souza, Emanuel M.; Pedrosa, Fabio O.; Comarella, Larissa; Irino, Kinue; Kato, Maria Aide M. F.; Farah, Sonia M. S. S.; Warth, Jose F.; Fadel-Picheth, Cyntia M. T.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/07/2008

Resumo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The presence of Shiga toxin-producing Escherichia coli (STEC) strains in feces samples of cattle was determined using the cytotoxicity assay on Vero cells and a screening PCR system to detect stx genes. The STEC isolates were sero-typed, tested for antimicrobial Susceptibility, and analyzed for virulence genes using multiplex PCR. The verocytotoxin-producing E. coli - reverse passive latex agglutination (VTEC-RPLA) assay was also used to detect Shiga toxin production. The frequency of cattle shedding STEC was 36%. The isolates belonged to 33 different serotypes. of which O10:H42, O98:H41, and O159:H21 had not previously been associated with STEC. The most frequent serotypes were ONT:H7 (10%), O22:H8 (7%) O22:H16 (7%), and ONT:H21 (7%). Most of the strains (96%) were susceptible to all antimicrobial agents tested. Shiga toxin was detected by the VTEC-RPLA assay in most (89%) of the STEC strains. The frequency of virulence markers was as follows: stx(1), 10%; stx(2), 43%; stx(1), plus stx(2), 47%; ehxA, 44%; eae, 1%; and saa, 38%. Several strains belong to serotypes associated with human disease, and most of them carried a stx(2)-type gene, suggesting that they represent a risk to human health. The screening PCR assay showed fewer false-negative results for STEC than the Vero-cell assay and is suitable for laboratory routine.

Formato

588-593

Identificador

http://dx.doi.org/10.1139/W08-046

Canadian Journal of Microbiology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 54, n. 7, p. 588-593, 2008.

0008-4166

http://hdl.handle.net/11449/3008

10.1139/W08-046

WOS:000258504800009

Idioma(s)

eng

Publicador

Natl Research Council Canada-n R C Research Press

Relação

Canadian Journal of Microbiology

Direitos

closedAccess

Palavras-Chave #STEC #stx genes #Virulence genes #Shiga toxin #antimicrobial susceptibility
Tipo

info:eu-repo/semantics/article