Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus


Autoria(s): Gibertoni, A. M.; Montassier, MDS; Sena, JAD; Givisiez, PEN; Furuyama, CRAG; Montassier, H. J.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/04/2005

Resumo

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

Formato

1982-1984

Identificador

http://dx.doi.org/10.1128/JCM.43.4.1982-1984.2005

Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1982-1984, 2005.

0095-1137

http://hdl.handle.net/11449/2876

10.1128/JCM.43.4.1982-1984.2005

WOS:000228404100081

WOS000228404100081.pdf

Idioma(s)

eng

Publicador

Amer Soc Microbiology

Relação

Journal of Clinical Microbiology

Direitos

openAccess

Tipo

info:eu-repo/semantics/article