Transcription of the Hsp30, Hsp70, and Hsp90 heat shock protein genes is modulated by the PalA protein in response to acid pH-sensing in the fungus Aspergillus nidulans
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/09/2011
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Heat shock proteins are molecular chaperones linked to a myriad of physiological functions in both prokaryotes and eukaryotes. In this study, we show that the Aspergillus nidulans hsp30 (ANID_03555.1), hsp70 (ANID_05129.1), and hsp90 (ANID_08269.1) genes are preferentially expressed in an acidic milieu, whose expression is dependent on the palA (+) background under optimal temperature for fungal growth. Heat shock induction of these three hsp genes showed different patterns in response to extracellular pH changes in the palA(+) background. However, their accumulation upon heating for 2 h was almost unaffected by ambient pH changes in the palA (-) background. The PalA protein is a member of a conserved signaling cascade that is involved in the pH-mediated regulation of gene expression. Moreover, we identified several genes whose expression at pH 5.0 is also dependent on the palA (+) background. These results reveal novel aspects of the heat- and pH-sensing networks of A. nidulans. |
Formato |
565-572 |
Identificador |
http://dx.doi.org/10.1007/s12192-011-0267-5 Cell Stress & Chaperones. Dordrecht: Springer, v. 16, n. 5, p. 565-572, 2011. 1355-8145 http://hdl.handle.net/11449/729 10.1007/s12192-011-0267-5 WOS:000293965500009 |
Idioma(s) |
eng |
Publicador |
Springer |
Relação |
Cell Stress & Chaperones |
Direitos |
closedAccess |
Palavras-Chave | #Aspergillus nidulans #pH sensing #pal signaling pathway #HSP |
Tipo |
info:eu-repo/semantics/article |