Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers: a powerful strategy for breast cancer diagnosis


Autoria(s): Silva, Catarina L.; Passos, Mário; Câmara, José de Sousa
Data(s)

10/12/2015

10/12/2015

01/01/2012

Resumo

A sensitive assay to identify volatile organic metabolites (VOMs) as biomarkers that can accurately diagnose the onset of breast cancer using non-invasively collected clinical specimens is ideal for early detection. Therefore the aim of this study was to establish the urinary metabolomic profile of breast cancer patients and healthy individuals (control group) and to explore the VOMs as potential biomarkers in breast cancer diagnosis at early stage. Solid-phase microextraction (SPME) using CAR/PDMS sorbent combined with gas chromatography–mass spectrometry was applied to obtain metabolomic information patterns of 26 breast cancer patients and 21 healthy individuals (controls). A total of seventy-nine VOMs, belonging to distinct chemical classes, were detected and identified in control and breast cancer groups. Ketones and sulfur compounds were the chemical classes with highest contribution for both groups. Results showed that excretion values of 6 VOMs among the total of 79 detected were found to be statistically different (p < 0.05). A significant increase in the peak area of (−)-4-carene, 3-heptanone, 1,2,4-trimethylbenzene, 2-methoxythiophene and phenol, in VOMs of cancer patients relatively to controls was observed. Statiscally significant lower abundances of dimethyl disulfide were found in cancer patients. Bioanalytical data were submitted to multivariate statistics [principal component analysis (PCA)], in order to visualize clusters of cases and to detect the VOMs that are able to differentiate cancer patients from healthy individuals. Very good discrimination within breast cancer and control groups was achieved. Nevertheless, a deep study using a larger number of patients must be carried out to confirm the results.

Identificador

Silva, C. L., Passos, M., & Câmara, J. S. (2012). Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers: a powerful strategy for breast cancer diagnosis. Talanta, 89, 360-368.

http://hdl.handle.net/10400.13/950

10.1016/j.talanta.2011.12.041

Idioma(s)

eng

Publicador

Elsevier

Relação

Portuguese Foundation for Science and Technology (FCT) through the Pluriannual base funding (QUI-Madeira-674) and Portuguese National Mass Spectrometry Network (REDE/1508/RNEM/2005)

Direitos

openAccess

Palavras-Chave #Breast cancer #Urine samples #Solid-phase microextraction #Volatile organic metabolites #. #Faculdade de Ciências Exatas e da Engenharia
Tipo

article