Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(ii) using common biological buffers as co-reactant, pH buffer and supporting electrolyte
Data(s) |
01/01/2015
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Resumo |
A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Royal Society of Chemistry |
Relação |
http://dro.deakin.edu.au/eserv/DU:30080557/francis-electrogenerated-2015.pdf http://www.dx.doi.org/10.1039/c5an01216c |
Direitos |
2015, Royal Society of Chemistry |
Palavras-Chave | #Science & Technology #Physical Sciences #Chemistry, Analytical #Chemistry #HYDROGEN-ION BUFFERS #SELECTIVE EXCITATION #ELECTROCHEMILUMINESCENCE #SYSTEMS #ECL #TRIS(2,2'-BIPYRIDINE)RUTHENIUM(II) #LUMINESCENCE #COMPLEXES #EMISSION #MONOMERS |
Tipo |
Journal Article |