Synthetic biology based metabolic engineering of saccharomyces cerevisiae for omega 6 and 3 PUFA production
| Contribuinte(s) |
Barrow, Colin Puri, Munish |
|---|---|
| Data(s) |
01/06/2015
|
| Resumo |
Metabolic engineering of PUFA biosynthesis pathway using codon optimized DGA1 (Diacylglycerol acyltransferase), FAA3 (Acyl-CoA synthetase), desaturase genes named D9D, D12D, D5D, D6D, D17D and D6E elongase gene was studied in S. cerevisiae. Engineered yeast strains successfully demonstrated increase in lipid accumulation, and heterologous biosynthesis of linoleic, γ-linolenic, dihomo γ-linolenic, arachidonic and eicosapentaenoic acid. |
| Identificador | |
| Idioma(s) |
eng |
| Publicador |
Deakin Univeristy, Faculty of Science, Engineering and Built Environment, School of Life and Environmental Sciences |
| Relação |
http://dro.deakin.edu.au/eserv/DU:30079026/sonkar-agreement-2015.pdf http://dro.deakin.edu.au/eserv/DU:30079026/sonkar-syntheticbiology-2015A.pdf |
| Direitos |
The author |
| Palavras-Chave | #metabolic engineering #polyunsaturated fatty acids (PUFAs) #sustainable PUFA sources #transgenic yeast |
| Tipo |
Thesis |
