Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection
Data(s) |
01/06/2015
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Resumo |
Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Wiley |
Relação |
http://dro.deakin.edu.au/eserv/DU:30074737/holland-screeningof-2015.pdf http://dro.deakin.edu.au/eserv/DU:30074737/pandohee-screeningof-inpress-2015.pdf http://www.dx.doi.org/10.1002/jssc.201500088 http://www.ncbi.nlm.nih.gov/pubmed/25845561 |
Direitos |
2015, Wiley |
Palavras-Chave | #2D chromatography #Cannabis #Offline chromatography #Profiling #Science & Technology #Physical Sciences #Chemistry, Analytical #Chemistry #MASS-SPECTROMETRY #SATIVA L #CONSTITUENTS #SEPARATIONS #ANTIOXIDANT #PRODUCTS #SOLVENT #CELL |
Tipo |
Journal Article |