Studies on the interaction between malanin and metal ions by fluorescence spectra methods
Data(s) |
01/12/2014
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Resumo |
A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37×10-4 and 2.66×10-4 mol·L-1, respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37×10-4 mol·L-1. Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation. |
Identificador | |
Idioma(s) |
chi |
Publicador |
Peking University Press |
Relação |
http://dro.deakin.edu.au/eserv/DU:30070978/yuan-studiesonthe-2014.pdf http://dro.deakin.edu.au/eserv/DU:30070978/yuan-studiesonthe-evid.pdf http://www.dx.doi.org/10.3964/j.issn.1000-0593(2014)12-3297-04 http://www.dx.doi.org/10.3964/j.issn.1000-0593(2014)12-3297-04 |
Direitos |
2014, Peking University Press |
Palavras-Chave | #Fluorescence quenching #Fluorescence spectrum #Malanin #Metal ion #Science & Technology #Technology #Spectroscopy #OXYGEN #VENOM |
Tipo |
Journal Article |