Detachment and flow cytometric quantification of seagrass-associated bacteria
Data(s) |
01/07/2014
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Resumo |
A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Relação |
http://www.dx.doi.org/10.1016/j.mimet.2014.04.008 http://www.ncbi.nlm.nih.gov/pubmed/24780150 |
Direitos |
2014, Elsevier |
Palavras-Chave | #Bacterial detachment #Enumeration #Flow cytometry #Microbe #Seagrass #Seagrass-associated bacteria |
Tipo |
Journal Article |