Theoretical modeling and experimental validation of surface stress in thrombin aptasensor
Data(s) |
01/12/2014
|
---|---|
Resumo |
Adsorption of target molecules on the immobilized microcantilever surface produced beam displacement due to the differential surface stress generated between the immobilized and non-immobilized surface. Surface stress is caused by the intermolecular forces between the molecules. Van der Waals, electrostatic forces, hydrogen bonding, hydrophobic effect and steric hindrance are some of the intermolecular forces involved. A theoretical framework describing the adsorption-induced microcantilever displacement is derived in this paper. Experimental displacement of thrombin aptamer-thrombin interactions was carried out. The relation between the electrostatic interactions involved between adsorbates (thrombin) as well as adsorbates and substrates (thrombin aptamer) and the microcantilever beam displacement utilizing the proposed mathematical model was quantified and compared to the experimental value. This exercise is important to aid the designers in microcantilever sensing performance optimization. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Institute of Electrical and Electronics Engineers Inc. |
Relação |
http://dro.deakin.edu.au/eserv/DU:30069941/lim-theoreticalmodeling-2014.pdf http://www.dx.doi.org/10.1109/TNB.2014.2337517 http://www.ncbi.nlm.nih.gov/pubmed/25122838 |
Direitos |
2014, Institute of Electrical and Electronics Engineers Inc. |
Palavras-Chave | #Atomistic #continuum #intermolecular forces #mathematical model #microcantilever #surface stress #Science & Technology #Life Sciences & Biomedicine #Biochemical Research Methods #Nanoscience & Nanotechnology #Biochemistry & Molecular Biology #Science & Technology - Other Topics #SELF-ASSEMBLED MONOLAYERS #MICROCANTILEVER SENSORS #DNA #BINDING #PROTEINS #RECONSTRUCTION #NANOMECHANICS #HYBRIDIZATION #RECOGNITION #MOLECULES |
Tipo |
Journal Article |