Multifunctional nanoparticle-EpCAM aptamer bioconjugates: a paradigm for targeted drug delivery and imaging in cancer therapy


Autoria(s): Das, M; Duan, W; Sahoo, SK
Data(s)

01/09/2014

Resumo

The promising proposition of multifunctional nanoparticles for cancer diagnostics and therapeutics has inspired the development of theranostic approach for improved cancer therapy. Moreover, active targeting of drug carrier to specific target site is crucial for providing efficient delivery of therapeutics and imaging agents. In this regard, the present study investigates the theranostic capabilities of nutlin-3a loaded poly (lactide-co-glycolide) nanoparticles, functionalized with a targeting ligand (EpCAM aptamer) and an imaging agent (quantum dots) for cancer therapy and bioimaging. A wide spectrum of in vitro analysis (cellular uptake study, cytotoxicity assay, cell cycle and apoptosis analysis, apoptosis associated proteins study) revealed superior therapeutic potentiality of targeted drug loaded NPs over other formulations in EpCAM expressing cells. Moreover, our nanotheranostic system served as a superlative bio-imaging modality both in 2D monolayer culture and tumor spheroid model. Our result suggests that, these aptamer-guided multifunctional NPs may act as indispensable nanotheranostic approach toward cancer therapy.

Identificador

http://hdl.handle.net/10536/DRO/DU:30067286

Idioma(s)

eng

Publicador

Elsevier

Relação

http://dro.deakin.edu.au/eserv/DU:30067286/duan-multifunctional-inpress-2014.pdf

http://www.dx.doi.org/10.1016/j.nano.2014.09.002

http://www.ncbi.nlm.nih.gov/pubmed/25240596

Direitos

2015, Elsevier

Palavras-Chave #EpCAM aptamer #Nanotheranostics #Nutlin-3a #PLGA NPs #Quantum dots
Tipo

Journal Article