Cloning and characterisation of the hint homologue of the Thermophile Thermus thermophilus
Data(s) |
01/01/2001
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Resumo |
Screening of a genomic library of the thermophile Thermus thermophilus revealed a novel thermophilic hint gene, homologues of which are highly conserved in genera from archaea to mammals. Hint belongs to the HIT protein super-family, which contains two broad groups, Fhit, associated with tumour suppression in eukaryotes and Hint with putatitive protein kinase C inhibitory activity. In T. thermophilus the 321bp gene has a GC content of 67% overall and 94.4% in the third nucleotide position, with unusually no thymine as a wobble base. The gene product, a small highly conserved 11996Da predicted soluble cytoplasmic protein, offers an ideal opportunity to investigate thermostabilising amino acid substitutions. Here we report on the characterisation of the novel hint sequence. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Informa Healthcare |
Relação |
http://dro.deakin.edu.au/eserv/DU:30063543/gibert-cloningand-2001.pdf http://doi.org/10.3109/10425170109080772 |
Direitos |
2001, Informa Healthcare |
Palavras-Chave | #Thermus thermophilus #hint #HIT family #protein kinase C inhibitor (PKCI) #thermostable |
Tipo |
Journal Article |