Granulocyte-macrophage colony-stimulating factor augments phagocytosis of mycobacterium avium complex by human immunodeficiency virus type 1-infected monocytes/macrophages in vitro and in vivo
Data(s) |
01/01/2000
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Resumo |
The role of human immunodeficiency virus type 1 (HIV-1) infection on the ability of human monocytes/macrophages to phagocytose Mycobacterium avium complex (MAC) in vivo and in vitro and the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on this function were investigated. By use of a flow cytometric assay to quantify phagocytosis, HIV-1 infection was found to impair the ability of monocyte-derived macrophages to phagocytose MAC in vitro, whereas GM-CSF significantly improved this defect. Phagocytosis was not altered by exposure to a mutant form of GM-CSF (E21R) binding only to the α chain of the GM-CSF receptor, suggesting that signaling by GM-CSF that leads to augmentation of phagocytosis is via the β chain of the receptor. In a patient with AIDS and disseminated multidrug-resistant MAC infection, GM-CSF treatment improved phagocytosis of MAC by peripheral blood monocytes and reduced bacteremia. These results imply that GM-CSF therapy may be useful in restoring antimycobacterial function by human monocytes/macrophages.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Oxford University Press |
Relação |
http://dro.deakin.edu.au/eserv/DU:30047514/mak-granulocytemacrophage-2000.pdf http://dx.doi.org/10.1086/315191 |
Direitos |
2000, Oxford University Press |
Palavras-Chave | #cultured cells #granulocyte-macrophage colony-stimulating factor #HIV infections #HIV-1 #macrophages #monocytes #mycobacterium avium complex #mycobacterium avium-intracellulare Infection #phagocytosis |
Tipo |
Journal Article |