DNA found in human immunodeficiency virus type 1 particles may not be required for infectivity
Data(s) |
01/08/1994
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Resumo |
We have studied the presence and significance of retroviral genome-derived DNA in the core of human immunodeficiency virus (HIV) particles produced from transfections of HXB2 expression vectors in COS-7 cells and from HIV type 1 IIIB chronically infected H9 cells. Viruses purified by sucrose cushion centrifugation and treated with DNase I contained 1000-fold more viral RNA than DNA. However protease-defective viruses that contained only pl60 ga~p°z had less than 100 times the amount of DNA in their cores than wild-type viruses suggesting that the p66/p51 form of reverse transcriptase was responsible for DNA transcription. Viruses produced by transfections in the presence of 3'-azido-3'-deoxythymidine (AZT) contained the viral RNA genome but only DNA of premature length because of the chain terminating effects of AZT. However such viruses were as infectious for CD4 + cells as wild-type virus. We conclude that retrovirus-derived DNA in HIV-1 particles is not required for infection and does not play a significant role in this process.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Society for General Microbiology |
Relação |
http://dro.deakin.edu.au/eserv/DU:30047512/arts-dnafound-1994.pdf http://dx.doi.org/10.1099/0022-1317-75-7-1605 |
Direitos |
1994, Society for General Microbiology |
Tipo |
Journal Article |