The solubility of Hen lysozyme in ethylammonium nitrate / H20 mixtures : a novel approach to protein crystallization
Data(s) |
04/02/2010
|
---|---|
Resumo |
We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydration of a high concentration protein solution. We describe the production of crystals of X-ray diffraction quality. Two related ionic liquid solvent systems, with the same solubility profiles but different effective pH characteristics, are identified for future evaluation.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
M D P I AG |
Relação |
http://dro.deakin.edu.au/eserv/DU:30039447/byrne-solubilityofhen-2010.pdf http://dx.doi.org/10.3390/molecules15020793 |
Direitos |
2010, M D P I AG |
Tipo |
Journal Article |