Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination
Data(s) |
01/08/2002
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Resumo |
RNA polymerase II (pol II) transcription termination requires co-transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted -propeller-forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C-terminal domain (CTD) of pol II in vitro and in a two-hybrid test in vivo. Furthermore, transcriptional run-on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3'-end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Nature Publishing Group |
Relação |
http://dro.deakin.edu.au/eserv/DU:30039331/dichtl-yhh1pcft1p-2002.pdf http://dx.doi.org/10.1093/emboj/cdf390 |
Direitos |
2002, Nature Publishing Group |
Palavras-Chave | #ß-propeller repeats #pre-mRNA 3'-end formation #RNA polymerase II C-terminal domain #RNA–protein interactions #transcription termination |
Tipo |
Journal Article |