Diversity and clonotypic composition of influenza-specific CD8+ TCR repertoires remain unaltered in the absence of Aire
Data(s) |
01/01/2010
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Resumo |
TCR repertoire diversity is important for the protective efficacy of CD8<sup>+</sup> T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted <i>versus</i> diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (D<sup>b</sup>NP<sub>366</sub>%<sup>+</sup>CD8<sup>+</sup>) and diverse (D<sup>b</sup>PA<sub>224</sub>%<sup>+</sup>CD8<sup>+</sup>, K<sup>d</sup>NP<sub>147</sub>%<sup>+</sup>CD8<sup>+</sup>) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, D<sup>b</sup>NP<sub>366</sub>%<sup>+</sup>CD8<sup>+</sup>, D<sup>b</sup>PA<sub>224</sub>%<sup>+</sup>CD8<sup>+</sup> and K<sup>d</sup>NP<sub>147</sub>%<sup>+</sup>CD8<sup>+</sup> T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Wiley - V C H Verlag GmbH & Co. KGaA |
Relação |
http://dro.deakin.edu.au/eserv/DU:30028854/stambas-diversityandclonotypic-2010.pdf http://dx.doi.org/10.1002/eji.200939918 |
Direitos |
2009, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Palavras-Chave | #CD8+ T cells #influenza #T-cell receptor repertoire #viral infection |
Tipo |
Journal Article |