Utilization of bacteriophage NCIMB-10645 of Serratia marcescens strain NCIMB-10644 as a tracer of pollution from an ocean sewage outlet

Bacteriophage NCIMB-10645 of Serratia marcescens (NCIMB-10644) was assessed as a tracer of aquatic contamination with the intention of using it at the Black Rock ocean outfall in South Eastern Australia. Growth conditions and a liquid culture growth curve were assessed. The bacteriophage was subjected to survival trials and a method of concentrating NCIMB-10645 developed. NCIMB-10645 was assessed as a tracer at Deakin University then released from the Black Rock Sewage Treatment Plant. Contamination movement away from the treatment plant was found to be predominantly affected by Easterly wind conditions.