Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development
Data(s) |
01/05/2008
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Resumo |
Merozoite surface protein 8 (MSP8) has shown promise as a vaccine candidate in the Plasmodium yoelii rodent malaria model and has a proposed role in merozoite invasion of erythrocytes. However, the temporal expression and localisation of MSP8 are unusual for a merozoite antigen. Moreover, in Plasmodium falciparum the MSP8 gene could be disrupted with no apparent effect on in vitro growth. To address the in vivo function of full-length MSP8, we truncated MSP8 in the rodent parasite Plasmodium berghei. PbΔMSP8 disruptant parasites displayed a normal blood-stage growth rate but no increase in reticulocyte preference, a phenomenon observed in P. yoelii MSP8 vaccinated mice. Expression levels of erythrocyte surface antigens were similar in P. berghei wild-type and PbΔMSP8-infected erythrocytes, suggesting that a parasitophorous vacuole function for MSP8 does not involve global trafficking of such antigens. These data demonstrate that a full-length membrane-associated form of PbMSP8 is not essential for blood-stage growth.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
http://dro.deakin.edu.au/eserv/DU:30017589/dekoning-truncationof-post-2008.pdf http://dro.deakin.edu.au/eserv/DU:30017589/dekoningward-truncationofplasmodium-2008.pdf http://dx.doi.org/10.1016/j.molbiopara.2008.01.005 |
Direitos |
2008, Elsevier B.V. |
Palavras-Chave | #malaria #Plasmodium berghei #merozoite surface proteins #MSP8 #gene knockout |
Tipo |
Journal Article |