A hybrid FIA/HPLC system incorporating monolithic column chromatography


Autoria(s): Adcock, Jacqui L.; Francis, Paul S.; Agg, Kent M.; Marshall, Graham D.; Barnett, Neil W.
Data(s)

02/09/2007

Resumo

We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2′-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.<br /><br />

Identificador

http://hdl.handle.net/10536/DRO/DU:30007499

Idioma(s)

eng

Publicador

Elsevier BV

Relação

http://dro.deakin.edu.au/eserv/DU:30007499/adcock-hybridfia-2007.pdf

http://dx.doi.org/10.1016/j.aca.2007.03.048

Direitos

2007, Elsevier B.V.

Palavras-Chave #flow injection analysis #gradient elution #monolithic column chromatography #chemiluminescence detection #neurotransmitter metabolites
Tipo

Journal Article