Rapid determination of papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection
Data(s) |
30/07/2007
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Resumo |
We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from <i>Papaver somniferum</i>. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids – morphine, codeine, oripavine and thebaine – were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 1 × 10<sup>−10 </sup>M, 5 × 10<sup>−10</sup> M, 5 × 10<sup>−10</sup> M and 1 × 10<sup>−9 </sup>M, for morphine, codeine, oripavine and thebaine, respectively. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier BV |
Relação |
http://dro.deakin.edu.au/eserv/DU:30007489/barnett-rapiddetermination-2007.pdf http://dx.doi.org/10.1016/j.aca.2007.06.044 |
Direitos |
2007, Elsevier B.V. |
Palavras-Chave | #monolithic column #alkaloids #chemiluminescence |
Tipo |
Journal Article |