The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2´-bipyridyl)ruthenium(II) chemiluminescence detection system
Data(s) |
01/01/2006
|
---|---|
Resumo |
This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C<sub>12</sub> column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 × 10<sup>−8 </sup>and 3.5 × 10<sup>−9 </sup>mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom. |
Identificador | |
Idioma(s) |
eng |
Publicador |
American Society for Testing and Materials |
Relação |
http://dro.deakin.edu.au/eserv/DU:30003790/lewis-determinationof-2006.pdf http://dro.deakin.edu.au/eserv/DU:30003790/lewis-determinationof-evidence-2006.pdf http://dx.doi.org/10.1111/j.1556-4029.2005.00033.x |
Direitos |
2006, ASTM International |
Palavras-Chave | #forensic science #HPLC #chemiluminescence detection #psilocin #psilocybin #hallucinogenic mushrooms #extraction |
Tipo |
Journal Article |