Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection


Autoria(s): Adcock, Jacqui L.; Barnett, Neil W.; Costin, Jason W.; Francis, Paul S.; Lewis, Simon W.
Data(s)

15/09/2005

Resumo

The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; α,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5 × 10<sup>−9</sup> and 4 × 10<sup>−8 </sup>M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine – without the need for extraction procedures – using monolithic column chromatography with chemiluminescence detection.

Identificador

http://hdl.handle.net/10536/DRO/DU:30003195

Idioma(s)

eng

Publicador

Elsevier BV

Relação

http://dro.deakin.edu.au/eserv/DU:30003195/adcock-determinationof-2005.pdf

http://dx.doi.org/10.1016/j.talanta.2005.03.007

Direitos

2005, Elsevier BV

Palavras-Chave #neurotransmitter metabolites #monolithic column chromatography; #chemiluminescence detection
Tipo

Journal Article