Preliminary evaluation of dual acidic potassium permanganate and tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection for the HPLC determination of Papaver somniferum alkaloids
Data(s) |
01/10/2004
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Resumo |
This paper describes a dual chemiluminescence reagent for the determination of the opiate alkaloids morphine, codeine, oripavine, and thebaine in <i>Papaver somniferum</i> extracts. Detection was achieved using a mixture of acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(ii), where the former acted as both the oxidant for the latter and as a chemiluminescence reagent in its own right. The analytes were separated on a C<sub>8</sub> column using ion-pairing HPLC. The application of the mixed reagent detection compared favourably with results obtained using standard HPLC methodology. Detection limits for the alkaloids were 10<sup>-6</sup>, 5 × 10<sup>-7</sup>, 3 × 10<sup>-6</sup>, and 2 × 10<sup>-6</sup> mol L<sup>-1</sup> for morphine, codeine, oripavine, and thebaine, respectively. |
Identificador | |
Idioma(s) |
eng |
Publicador |
CSIRO Publishing |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002785/barnett-preliminaryevolution-2004.pdf http://dx.doi.org/10.1071/CH04084 |
Direitos |
2004, CSIRO Publishing |
Tipo |
Journal Article |