Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection
Data(s) |
15/11/2004
|
---|---|
Resumo |
Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10<sup>−6</sup> M) and the detection limit was 1 × 10<sup>−8</sup> M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy)<sub>3</sub><sup>3+</sup> consumption and its associated effect on accuracy. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier BV |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002783/barnett-determinationof-2004.pdf http://dx.doi.org/10.1016/j.talanta.2004.03.065 |
Direitos |
2004, Elsevier B.V. |
Palavras-Chave | #tris(2,2′-bipyridyl)ruthenium(II) #chemiluminescence; #Ru(bipy)33+ #flow injection analysis #proline #wine |
Tipo |
Journal Article |