Effect of exercise on protein kinase C activity and localization in human skeletal muscle
Data(s) |
01/12/2004
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Resumo |
To investigate the effect of exercise on protein kinase C (PKC) activity and localization in human skeletal muscle, eight healthy men performed cycle ergometer exercise for 40 min at 76±1% the peak pulmonary O<sub>2</sub> uptake (V<sub>O2peak</sub>), with muscle samples obtained at rest and after 5 and 40 min of exercise. PKC expression, phosphorylation and activities were examined by immunoblotting and <i>in vitro</i> kinase assays of fractionated and whole tissue preparations. In response to exercise, total PKC activity was slightly higher at 40 min in an enriched membrane fraction, and using a pSer-PKC-substrate motif antibody it was revealed that exercise increased the serine phosphorylation of a ∼50 kDa protein. There were no changes in conventional PKC (cPKC) or PKC<i>θ</i> activities; however, atypical PKC (aPKC) activity was ∼70% higher at 5 and 40 min, and aPKC expression and Thr<sup>410/403</sup> phosphorylation were unaltered by exercise. There were no effects of exercise on the abundance of PKCα, PKCδ, PKC<i>θ</i> and aPKC within cytosolic or enriched membrane fractions of skeletal muscle. These data indicate that aPKC, but not cPKC or PKC<i>θ</i>, are activated by exercise in contracting muscle suggesting a potential role for aPKC in the regulation of skeletal muscle function and metabolism during exercise in humans.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Blackwell Publishing Ltd |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002437/n20040285.pdf http://dx.doi.org/10.1113/jphysiol.2004.075549 |
Direitos |
2004, The Physiological Society |
Tipo |
Journal Article |