Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs
Data(s) |
01/02/2002
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Resumo |
Na<sup>+</sup>/H<sup>+</sup> exchangers are integral membrane proteins that exchange Na<sup>+</sup> and H<sup>+</sup> across cell membranes. The Na<sup>+</sup>/H<sup>+</sup> exchangers 2 and 3 are epithelial isoforms in mammals and contribute to acid–base homeostasis. The gills of fishes, including elasmobranchs, are also associated with acid/base balance, and are probably the primary acid/base regulatory organ. This study examines the presence of Na<sup>+</sup>/H<sup>+</sup> exchangers 2 and 3 using immunohistochemistry and immunoblotting in the gills of four species of elasmobranchs, the banjo ray (Trygonorrhina fasciata), southern eagle ray (<i>Myliobatis australis</i>), the gummy shark (<i>Mustelus antarcticus</i>) and the Australian angel shark (<i>Squatina australis</i>) using heterologous antibodies. Na<sup>+</sup>/H<sup>+</sup> exchanger 2-like immunoreactivity was observed in the gills of the banjo ray, eagle ray and angel shark. In the banjo and eagle rays, this Na<sup>+</sup>/H<sup>+</sup> exchanger-like immunoreactivity co-localised with immunoreactivity to Na<sup>+</sup>/K<sup>+</sup>-ATPase, a marker for the mitochondrial-rich cells of fishes. Na<sup>+</sup>/H<sup>+</sup> exchanger 3-like immunoreactivity was only observed in the gills of the angel and gummy sharks, some Na<sup>+</sup>/H<sup>+</sup> exchanger 3-like cells also showed Na<sup>+</sup>/K<sup>+</sup>-ATPase immunoreactivity. However, immunoblotting of banjo and eagle ray gill membranes demonstrated Na<sup>+</sup>/H<sup>+</sup> exchanger 3-like immunoreactivity, which was not consistent with the immunohistochemical results. These data demonstrate the presence of epithelial Na<sup>+</sup>/H<sup>+</sup> exchangers 2 and 3 in the gills of elasmobranchs and a link with acid/base regulation is suggested.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier Science Inc. |
Relação |
http://dro.deakin.edu.au/eserv/DU:30001696/n20020885.pdf http://dx.doi.org/10.1016/S1095-6433(01)00449-4 |
Direitos |
2002, Elsevier Science Inc |
Palavras-Chave | #acid/base regulation #elasmobranch #gills #sodium/proton exchanger #Na+/H+ exchanger (NHE) #mitochondria-rich cells |
Tipo |
Journal Article |