Immobilized analogues of sunflower trypsin inhibitor-1 constitute a versatile group of affinity sorbents for selective isolation of serine proteases


Autoria(s): PEREIRA, Hugo Juarez Vieira; SALGADO, Maria Cristina Oliveira; OLIVEIRA, Eduardo Brandt
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Sunflower trypsin inhibitor-1 (SFI-1), a natural 14-residue cyclic peptide, and some of its synthetic acyclic variants are potent protease inhibitors displaying peculiar inhibitory profiles. Here we describe the synthesis and use of affinity sorbents prepared by coupling SFTI-1 analogues to agarose resin. Chymotrypsinand trypsin-like proteases could then be selectively isolated from pancreatin; similarly, other proteases were obtained from distinct biological sources. The binding capacity of [Lys5]-SFTI-1-agarose for trypsin was estimated at over 10 mg/mL of packed gel. SFTI-1-based resins could find application either to improve the performance of current purification protocols or as novel protease-discovery tools in different areas of biological investigation. (C) 2009 Elsevier B.V. All rights reserved.

FAPESP Fundacao de Amparo a Pesquisa do Estado cle Sjo Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificador

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.877, n.22, p.2039-2044, 2009

1570-0232

http://producao.usp.br/handle/BDPI/31842

10.1016/j.jchromb.2009.05.036

http://dx.doi.org/10.1016/j.jchromb.2009.05.036

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Proteolytic enzymes #Serine proteases #Affinity chromatography #SFTI-1 #Bowman-Birk inhibitors #ARTERIAL BED PERFUSATE #SUBSTRATE-SPECIFICITY #POTENT INHIBITORS #REACTIVE-SITE #SFTI-1 #SEEDS #CHROMATOGRAPHY #ELASTASE-2 #MATRIPTASE #DESIGN #Biochemical Research Methods #Chemistry, Analytical
Tipo

article

original article

publishedVersion