Amperometric detection of benzoyl peroxide in pharmaceutical preparations using carbon paste electrodes with peroxidases naturally immobilized on coconut fibers
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
20/10/2012
20/10/2012
2010
|
Resumo |
This paper describes the applications of anew carbon paste electrode containing fibers of coconut (Cocus nucifera L) fruit, which are very rich in peroxidase enzymes naturally immobilized on its structure. The new sensor was applied for the amperometric quantification of benzoyl peroxide in facial creams and dermatological shampoos. The amperometric measurements were performed in 0.1 mol L(-1) phosphate buffer (pH 5.2), at 0.0 V (versus Ag/AgCl). On these conditions, benzoyl peroxide was rapidly determined in the 5.0-55 mu mol L(-1), with a detection limit of 2.5 mu mol L(-1) (s/n = 3), response time of 4.1 s (90% of the steady state) and sensitivity limit of 0.33 A mol L(-1) cm(-2). The amperometric results are in good agreement with those obtained by spectrophotometric technique, used as a standard method. (C) 2009 Elsevier B.V. All rights reserved. Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) IM2C (Instituto do Milenio de Materiais Complexos) IM2C (Instituto do Milenio de Materiais Complexos) Financiadora de Estudos e Projetos (FINEP) CTPetro SAMUTI SAMUTI |
Identificador |
BIOSENSORS & BIOELECTRONICS, v.25, n.5, p.1143-1148, 2010 0956-5663 http://producao.usp.br/handle/BDPI/31107 10.1016/j.bios.2009.09.044 |
Idioma(s) |
eng |
Publicador |
ELSEVIER ADVANCED TECHNOLOGY |
Relação |
Biosensors & Bioelectronics |
Direitos |
restrictedAccess Copyright ELSEVIER ADVANCED TECHNOLOGY |
Palavras-Chave | #Plant-tissue based biosensor #Amperometry #Peroxidase #Benzoyl peroxide #FLOW-INJECTION ANALYSIS #HYDROGEN-PEROXIDE #HORSERADISH-PEROXIDASE #LIQUID-CHROMATOGRAPHY #ENZYME ELECTRODE #TISSUE #BIOSENSORS #SENSORS #REACTOR #PHENOL #Biophysics #Biotechnology & Applied Microbiology #Chemistry, Analytical #Electrochemistry #Nanoscience & Nanotechnology |
Tipo |
article original article publishedVersion |