Molecular characterization of nitrate reductase gene and its expression in the marine red alga Gracilaria tenuistipitata (Rhodophyta)
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2010
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Resumo |
The enzyme nitrate reductase (NR) responsible for the conversion of nitrate to nitrite is considered to be the rate-limiting step in nitrogen assimilation. The economically important marine macroalga Gracilaria tenuistipitata presents a circadian oscillation in NR protein content and activity. In order to identify if the regulation of NR in G. tenuistipitata happens at transcriptional levels, the NR cDNA and gene were sequenced and the NR mRNA expression was studied. Analysis of the sequenced gene revealed absence of introns which is unusual for NR genes. The transcriptional profiling revealed a circadian rhythm for NR; furthermore, a rhythm was observed in constant light condition, suggesting a possible regulation by the biological clock at the mRNA levels for NR in G. tenuistipitata. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
Identificador |
JOURNAL OF APPLIED PHYCOLOGY, v.22, n.5, p.613-622, 2010 0921-8971 http://producao.usp.br/handle/BDPI/30962 10.1007/s10811-010-9501-2 |
Idioma(s) |
eng |
Publicador |
SPRINGER |
Relação |
Journal of Applied Phycology |
Direitos |
restrictedAccess Copyright SPRINGER |
Palavras-Chave | #Biological rhythm #Phylogeny #Gene expression #Gracilaria tenuistipitata #Nitrate reductase #ENCODING GENE #HIGHER-PLANTS #TRANSCRIPTIONAL REGULATION #PHYLOGENETIC TREES #AMINO-ACIDS #TOBACCO #ARABIDOPSIS #SEQUENCES #EVOLUTION #TOMATO #Biotechnology & Applied Microbiology #Marine & Freshwater Biology |
Tipo |
article original article publishedVersion |