Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818)
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
20/10/2012
20/10/2012
2008
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| Resumo |
The single cell gel eletrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5. 5, 10 and 20 Gy of Co-60 gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens. (c) 2008 Elsevier B.V. All rights reserved. |
| Identificador |
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v.654, n.1, p.58-63, 2008 1383-5718 http://producao.usp.br/handle/BDPI/30532 10.1016/j.mrgentox.2008.05.007 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER SCIENCE BV |
| Relação |
Mutation Research-genetic Toxicology and Environmental Mutagenesis |
| Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE BV |
| Palavras-Chave | #Biomphalaria #freshwater snail #DNA damage #single cell gel/comet assay #ionizing radiation #DETECTING DNA-DAMAGE #GAMMA-RADIATION #IONIZING-RADIATION #INDUSTRIAL-WASTES #HEMOLYMPH CELLS #GENOTOXICITY #MARINE #INVERTEBRATES #MICRONUCLEUS #HEMOCYTES #Biotechnology & Applied Microbiology #Genetics & Heredity #Toxicology |
| Tipo |
article original article publishedVersion |
