Effect of low-level laser irradiation on odontoblast-like cells
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2008
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Resumo |
Low-level laser therapy (LLLT), also referred to as therapeutic laser, has been recommended for a wide array of clinical procedures, among which the treatment of dentinal hypersensitivity. However, the mechanism that guides this process remains unknown. Therefore, the objective of this study was to evaluate in vitro the effects of LLL irradiation on cell metabolism (MTT assay), alkaline phosphatase (ALP) expression and total protein synthesis. The expression of genes that encode for collagen type-1 (Col-1) and fibronectin (FN) was analyzed by RT-PCR. For such purposes, oclontoblast-like cell line (MDPC-23) was previously cultured in Petri dishes (15000 cells/cm(2)) and submitted to stress conditions during 12 h. Thereafter, 6 applications with a monochromatic near infrared radiation (GaAlAs) set at predetermined parameters were performed at 12-h intervals. Non-irradiated cells served as a control group. Neither the MTT values nor the total protein levels of the irradiated group differed significantly from those of the control group (Mann-Whitney test; p > 0.05). On the other hand, the irradiated cells showed a decrease in ALP activity (Mann-Whitney test; p < 0.05). RT-PCR results demonstrated a trend to a specific reduction in gene expression after cell irradiation, though not significant statistically (Mann-Whitney test; p > 0.05). It may be concluded that, under the tested conditions, the LLLT parameters used in the present study did not influence cell metabolism, but reduced slightly the expression of some specific proteins. |
Identificador |
LASER PHYSICS LETTERS, v.5, n.9, p.680-685, 2008 1612-2011 http://producao.usp.br/handle/BDPI/30203 10.1002/lapl.200810042 |
Idioma(s) |
eng |
Publicador |
WILEY-V C H VERLAG GMBH |
Relação |
Laser Physics Letters |
Direitos |
restrictedAccess Copyright WILEY-V C H VERLAG GMBH |
Palavras-Chave | #doses #infrared lasers #odontoblast-like cells #TITANIUM IMPLANT MATERIAL #GLASS-IONOMER CEMENTS #IN-VITRO CYTOTOXICITY #ER-YAG LASER #PROMOTES PROLIFERATION #DENTAL PAPILLAE #GAALAS LASER #THERAPY #LINE #FIBROBLASTS #Instruments & Instrumentation |
Tipo |
article original article publishedVersion |