Autofluorescence spectroscopy in liver transplantation: Preliminary results from a pilot clinical study
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2008
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Resumo |
The evaluation of graft function at various stages after transplantation is relevant, particularly at the moment of organ harvest, when a decision must be made whether to use the organ. Autofluorescence spectroscopy is noninvasive technique to monitor the metabolic condition of a liver graft throughout its course, from an initial evaluation in the donor, through cold ischemia transportation, to reperfusion and reoxygenation in the recipient. Preliminary results are presented in six liver transplantations spanning the periods from liver harvest to implant. The laser-induced fluorescence spectrum at 532-mn excitation was investigated before cold perfusion (autofluorescence), during cold ischemia, at the back table procedure, as well as 5 and 60 minutes after reperfusion. The results showed that the fluorescence analysis was sensitive to changes during the transplantation procedure. Fluorescence spectroscopy potentially provides a real-time, noninvasive technique to monitor liver graft function. The information could potentially be valuable for surgical decisions and transplant success. |
Identificador |
TRANSPLANTATION PROCEEDINGS, v.40, n.3, p.722-725, 2008 0041-1345 http://producao.usp.br/handle/BDPI/29796 10.1016/j.transproceed.2008.03.005 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE INC |
Relação |
Transplantation Proceedings |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE INC |
Palavras-Chave | #REDUCED PYRIDINE NUCLEOTIDE #LIVING CELLS #FLUORESCENCE #LOCALIZATION #NADH #Immunology #Surgery #Transplantation |
Tipo |
article proceedings paper publishedVersion |