Influence of pH on the phototransformation process of PhotogemA (R)


Autoria(s): MENEZES, P. F. C.; IMASATO, H.; BAGNATO, Vanderlei Salvador; SIBATA, Cl. H.; PERUSSI, J. R.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

PhotogemA (R) is a hematoporphyrin derivative that has been used as a photosensitizer in experimental and clinical Photodynamic Therapy (PDT) in Brazil. Photosensitizers are degraded under illumination. This process, usually called photobleaching, can be monitored by decreasing in fluorescence intensities and includes the following photoprocesses: photodegradation, phototransformation, and photorelocalization. Photobleaching of hematoporphyrin-type sensitizers during illumination in aqueous solution is related not only to photodegradation but is also followed by the formation of photoproducts with a new fluorescence band at around 640-650 nm and with increased light absorption in the red spectral region at 640 nm. In this study, the influence of pH on the phototransformation process was investigated. PhotogemA (R) solutions, 40 mu g/ml, were irradiated at 514 nm with intensity of 100 mW/cm(2) for 20 min with different pH environments. The controls were performed with the samples in the absence of light. The PhotogemA (R) photodegradation is dependent on the pH. The behavior of photodegradation and photoproducts formation (monitored at 640 nm) is distinct and depends on the photosensitizer concentration. The processes of degradation and photoproducts formation were monitored with Photogemin the concentration of 40 mu g/mL since that demonstrated the best visualization of both processes. While below pH 5 the photodegradation occurred, there was no detectable presence of photoproducts. The increase of pH led to increase of photoproducts formation rate with photodegradation reaching the highest value at pH 10. The increase of photoproducts formation and instability of PhotogemA (R) from pH 6 to pH 10 are in agreement with the desired properties of an ideal photosensitizer since there are significant differences in pH between normal (7.0 < pH < 8.6) and tumor (5.8 < pH < 7.9) tissues. It is important to know the effect of pH in the process of phototransformation (degradation and photoproduct formation) of the molecule since low pH values promotes increase in the proportion of aggregates species in solution and high pH values promotes increase in the proportion of monomeric species. There must be an ideal pH interval which favors the phototransformation process that is correlated with the singlet oxygen formation responsible by the photodynamic effect. These differences in pH between normal and tumor cells can explain the presence of photosensitizers in target tumor cells, making PDT a selective therapy.

FAPESP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificador

LASER PHYSICS, v.19, n.7, p.1457-1462, 2009

1054-660X

http://producao.usp.br/handle/BDPI/29691

10.1134/S1054660X09070159

http://dx.doi.org/10.1134/S1054660X09070159

Idioma(s)

eng

Publicador

MAIK NAUKA/INTERPERIODICA/SPRINGER

Relação

Laser Physics

Direitos

restrictedAccess

Copyright MAIK NAUKA/INTERPERIODICA/SPRINGER

Palavras-Chave #PHOTODYNAMIC THERAPY #HEMATOPORPHYRIN DERIVATIVES #PHOTOPRODUCT FORMATION #PORPHYRINS #SENSITIZERS #FLUORESCENCE #CELLS #PHOTOSENSITIZERS #CYTOTOXICITY #DOSIMETRY #Optics #Physics, Applied
Tipo

article

original article

publishedVersion