Investigation of the Europium Emission Spectra of the Europium-Oxytetracycline Complex in the Presence of Human Low-Density Lipoproteins
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2011
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Resumo |
Low-Density Lipoprotein (LDL), often known as ""bad cholesterol"" is one of the responsible to increase the risk of coronary arterial diseases. For this reason, the cholesterol present in the LDL particle has become one of the main parameters to be quantified in routine clinical diagnosis. A number of tools are available to assess LDL particles and estimate the cholesterol concentration in the blood. The most common methods to quantify the LDL in the plasma are the density gradient ultracentrifugation and nuclear magnetic resonance (NMR). However, these techniques require special equipments and can take a long time to provide the results. In this paper, we report on the increase of the Europium emission in Europium-oxytetracycline complex aqueous solutions in the presence of LDL. This increase is proportional to the LDL concentration in the solution. This phenomenum can be used to develop a method to quantify the number of LDL particles in a sample. A comparison between the performances of the oxytetracycline and the tetracycline in the complexes is also made. CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP INCT-FCx (Instituto Nacional de Ciencias e Tecnologia de Fluidos Complexos) INCT-FCx (Instituto Nacional de Ciencias e Tecnologia de Fluidos Complexos) |
Identificador |
JOURNAL OF FLUORESCENCE, v.21, n.3, p.887-892, 2011 1053-0509 http://producao.usp.br/handle/BDPI/29161 10.1007/s10895-010-0602-8 |
Idioma(s) |
eng |
Publicador |
SPRINGER/PLENUM PUBLISHERS |
Relação |
Journal of Fluorescence |
Direitos |
restrictedAccess Copyright SPRINGER/PLENUM PUBLISHERS |
Palavras-Chave | #LDL #Europium #Fluorescence #Biosensor #GRADIENT GEL-ELECTROPHORESIS #CORONARY-ARTERY-DISEASE #ANALYTIC ULTRACENTRIFUGATION #TETRACYCLINE COMPLEX #FLUORESCENT-PROBE #HYDROGEN-PEROXIDE #LDL #SUBFRACTIONS #CHOLESTEROL #SYSTEM #Biochemical Research Methods #Chemistry, Analytical |
Tipo |
article original article publishedVersion |