Naturally acquired inhibitory antibodies to Plasmodium vivax Duffy binding protein are short-lived and allele-specific following a single malaria infection
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
The Duffy binding protein of Plasmodium vivax (DBP) is a critical adhesion ligand that participates in merozoite invasion of human Duffy-positive erythrocytes. A small outbreak of P. vivax malaria, in a village located in a non-malarious area of Brazil, offered us an opportunity to investigate the DBP immune responses among individuals who had their first and brief exposure to malaria. Thirty-three individuals participated in the five cross-sectional surveys, 15 with confirmed P. vivax infection while residing in the outbreak area (cases) and 18 who had not experienced malaria (non-cases). In the present study, we found that only 20% (three of 15) of the individuals who experienced their first P. vivax infection developed an antibody response to DBP; a secondary boosting can be achieved with a recurrent P. vivax infection. DNA sequences from primary/recurrent P. vivax samples identified a single dbp allele among the samples from the outbreak area. To investigate inhibitory antibodies to the ligand domain of the DBP (cysteine-rich region II, DBP(II)), we performed in vitro assays with mammalian cells expressing DBP(II) sequences which were homologous or not to those from the outbreak isolate. In non-immune individuals, the results of a 12-month follow-up period provided evidence that naturally acquired inhibitory antibodies to DBP(II) are short-lived and biased towards a specific allele. Brazilian National Research Council (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundacao Oswaldo Cruz, FIOCRUZ, PAPES IV[CNPq-400086/2006-9] Fundação Oswaldo Cruz (Fiocruz) Fundacao de Amparo a Pesquisa de Minas Gerais[FAPEMIG-CBB-880/06] Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) |
Identificador |
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, v.156, n.3, p.502-510, 2009 0009-9104 http://producao.usp.br/handle/BDPI/28729 10.1111/j.1365-2249.2009.03931.x |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL PUBLISHING, INC |
Relação |
Clinical and Experimental Immunology |
Direitos |
restrictedAccess Copyright WILEY-BLACKWELL PUBLISHING, INC |
Palavras-Chave | #allele-specific #antibody response #duffy binding protein #malaria #Plasmodium vivax #MEROZOITE SURFACE PROTEIN-1 #POLYMERASE-CHAIN-REACTION #LIGAND DOMAIN #COMPARATIVE RECOGNITION #ERYTHROCYTE INVASION #BRAZILIAN AMAZON #RECEPTOR #KNOWLESI #EXPOSURE #RELAPSES #Immunology |
Tipo |
article original article publishedVersion |