A vaccine against S. pyogenes: Design and experimental immune response
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Streptococcus pyogenes causes severe invasive infections: the post-streptococcal sequelae of acute rheumatic fever (RF) and rheumatic heart disease (RHD), acute glomerulonephritis, and uncomplicated pharyngitis and pyoderma. Efforts to produce a vaccine against S. pyogenes began several decades ago, and different models have been proposed. Here, we describe the methodology used in the development of a new vaccine model, consisting of both T and B protective epitopes constructed as synthetic peptides and recombinant proteins. Two adjuvants were tested in an experimental inbred mouse model: a classical Freund`s adjuvant and a new adjuvant (AFCo1) that induces mucosal immune responses and is obtained by calcium precipitation of a proteoliposome derived from the outer membrane of Neisseria meningitides B. The StreptInCor vaccine epitope co-administrated with AFCo1 adjuvant induced mucosal (IgA) and systemic (IgG) antibodies as preferential Th1-mediated immune responses. No autoimmune reactions were observed, suggesting that the vaccine epitope is safe. (c) 2009 Elsevier Inc. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) ""Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)"" from Brazil Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
Identificador |
METHODS, v.49, n.4, p.316-321, 2009 1046-2023 http://producao.usp.br/handle/BDPI/28707 10.1016/j.ymeth.2009.03.024 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
Methods |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #S. pyogenes #M protein #Vaccine #Autoimmunity #Adjuvant #Experimental assays #Immune response #Antibodies #Cytokines #A STREPTOCOCCAL VACCINE #RHEUMATIC HEART-DISEASE #M-PROTEIN #FEVER #IMMUNOGENICITY #ANTIBODIES #PROGRESS #Biochemical Research Methods #Biochemistry & Molecular Biology |
Tipo |
article original article publishedVersion |