Involvement of proteinase-activated receptors 1 and 2 in spreading and phagocytosis by murine adherent peritoneal cells: Modulation by the C-terminal of S100A9 protein


Autoria(s): PAGANO, Rosana L.; SAMPAIO, Sandra C.; JULIANO, Maria A.; JULIANO, Luiz; GIORGI, Renata
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

Proteinase-activated receptors (PAR) are widely recognized for their modulatory properties in inflammatory and immune responses; however, their direct role on phagocyte effector functions remains unknown. S100A9, a protein secreted during inflammatory responses, deactivates activated peritoneal macrophages, and its C-terminal portion inhibits spreading and phagocytosis of adherent peritoneal cells. Herein, the effect of PAR1 and PAR2 agonists was investigated on spreading and phagocytosis by adherent peritoneal cells, as well as the ability of murine C-terminal of S100A9 peptide (mS100A9p) to modulate this effect. Adherent peritoneal cells obtained from mouse abdominal cavity were incubated with PAR1 and PAR2 agonists and spreading and phagocytosis of Candida albicans particles were evaluated. PAR1 agonists increased both the spreading and the phagocytic activity, but PAR2 agonists only increased the spreading index. mS100A9p reverted both the increased spreading and phagocytosis induced by PAR1 agonists, but no interference in the increased spreading induced by PAR2 agonists was noticed. The shorter homologue peptide to the C-terminal of mS100A9p, corresponding to the H(92)-E(97) region, also reverted the increased spreading and phagocytosis induced by PAR1 agonists. These findings show that proteinase-activated receptors have an important role for spreading and phagocytosis of adherent peritoneal cells, and that the pepticle corresponding to the C-terminal of S100A9 protein is a remarkable candidate for use as a novel compound to modulate PAR1 function. (C) 2009 Elsevier B.V. All rights reserved.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP)

Fundação Butantan

Fundacao Butantan

Identificador

EUROPEAN JOURNAL OF PHARMACOLOGY, v.628, n.1/Mar, p.240-246, 2010

0014-2999

http://producao.usp.br/handle/BDPI/28697

10.1016/j.ejphar.2009.11.033

http://dx.doi.org/10.1016/j.ejphar.2009.11.033

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

European Journal of Pharmacology

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Proteinase-activated receptor #Adherent peritoneal cell #Phagocytosis #Spreading #S100A9 #(Mouse) #INHIBITS HYPERALGESIA #THROMBIN #BINDING #INFLAMMATION #NEUTROPHILS #MRP-14 #EXPRESSION #MONOCYTES #ADHESION #AGONIST #Pharmacology & Pharmacy
Tipo

article

original article

publishedVersion