The importance of hormone receptor analysis in osteosarcoma cells growth submitted to treatment with estrogen in association with thyroid hormone


Autoria(s): SARAIVA, Patricia Pinto; TEIXEIRA, Silvania Silva; CONDE, Sandro Jose; NOGUEIRA, Celia Regina
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2008

Resumo

Bone tumor incidence in women peaks at age 50-60, coinciding with the menopause. That estrogen (E2) and triiodothyronine (T3) interact in bone metabolism has been well established. However, few data on the action of these hormones are available. Our purpose was to determine the role of E2 and T3 in the expression of bone activity markers, namely alkaline phosphatase (AP) and receptor activator of nuclear factor kappa B ligand (RANKL). Two osteosarcoma cell lines: MG-63 (which has both estrogen (ER) and thyroid hormone (TR) receptors) and SaOs-29 (ER receptors only) were treated with infraphysiological E2 associated with T3 at infraphysiological, physiological, and supraphysiological concentrations. Real-time RT-PCR was used for expression analysis. Our results show that, in MG-63 cells, infraphysiological E2 associated with supraphysiological T3 increases AP expression and decreases RANKL expression, while infraphysiological E2 associated with either physiological or supraphysiological T3 decreases both AP and RANKL expression. On the other hand, in SaOs-2 cells, the same hormone combinations had no significant effect on the markers` expression. Thus, the analysis of hormone receptors was shown to be crucial for the assessment of tumor potential growth in the face of hormonal changes. Special care should be provided to patients with T3 and E2 hormone receptors that may increase tumor growth. Copyright (C) 2007 John Wiley & Sons, Ltd.

Identificador

CELL BIOCHEMISTRY AND FUNCTION, v.26, n.1, p.107-110, 2008

0263-6484

http://producao.usp.br/handle/BDPI/28646

10.1002/cbf.1408

http://dx.doi.org/10.1002/cbf.1408

Idioma(s)

eng

Publicador

JOHN WILEY & SONS LTD

Relação

Cell Biochemistry and Function

Direitos

restrictedAccess

Copyright JOHN WILEY & SONS LTD

Palavras-Chave #estrogen #thyroid hormone #hormone receptors #MG-63 #SaOs-2 #bone #RANKL #alkaline phosphatase #BONE METABOLISM #EXPRESSION #CANCER #LINES #17-BETA-ESTRADIOL #DIFFERENTIATION #INTERLEUKIN-6 #ESTRADIOL #BREAST #GENES #Biochemistry & Molecular Biology #Cell Biology
Tipo

article

original article

publishedVersion