The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus


Autoria(s): BUISSA-FILHO, R.; PUCCIA, R.; MARQUES, A. F.; PINTO, F. A.; MUNOZ, J. E.; NOSANCHUK, J. D.; TRAVASSOS, L. R.; TABORDA, C. P.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2008

Resumo

The protective role of specific antibodies against Paracoccidioides brasiliensis is controversial. In the present study, we analyzed the effects of monoclonal antibodies on the major diagnostic antigen (gp43) using in vitro and in vivo P. brasiliensis infection models. The passive administration of some monoclonal antibodies (MAbs) before and after intratracheal or intravenous infections led to a reduced fungal burden and decreased pulmonary inflammation. The protection mediated by MAb 3E, the most efficient MAb in the reduction of fungal burden, was associated with the enhanced phagocytosis of P. brasiliensis yeast cells by J774.16, MH-S, or primary macrophages. The ingestion of opsonized yeast cells led to an increase in NO production by macrophages. Passive immunization with MAb 3E induced enhanced levels of gamma interferon in the lungs of infected mice. The reactivity of MAb 3E against a panel of gp43-derived peptides suggested that the sequence NHVRIPIGWAV contains the binding epitope. The present work shows that some but not all MAbs against gp43 can reduce the fungal burden and identifies a new peptide candidate for vaccine development.

Identificador

INFECTION AND IMMUNITY, v.76, n.7, p.3321-3328, 2008

0019-9567

http://producao.usp.br/handle/BDPI/28616

10.1128/IAI.00349-08

http://dx.doi.org/10.1128/IAI.00349-08

Idioma(s)

eng

Publicador

AMER SOC MICROBIOLOGY

Relação

Infection and Immunity

Direitos

restrictedAccess

Copyright AMER SOC MICROBIOLOGY

Palavras-Chave #CRYPTOCOCCUS-NEOFORMANS INFECTION #PULMONARY PARACOCCIDIOIDOMYCOSIS #PASSIVE ANTIBODY #NITRIC-OXIDE #CELL #GLYCOPROTEIN #EFFICACY #SUSCEPTIBILITY #MACROPHAGES #IDENTIFICATION #Immunology #Infectious Diseases
Tipo

article

original article

publishedVersion