Expression and characterization of LTx2, a neurotoxin from Lasiodora sp effecting on calcium channels
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
20/10/2012
20/10/2012
2008
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| Resumo |
Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels. (C) 2008 Elsevier Inc. All rights reserved. Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)[CBB 718/05] Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) PRONEX[CBB APQ-4615-4.01/07] CNPq Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Process[471080/2006-3] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP |
| Identificador |
PEPTIDES, v.29, n.9, p.1505-1513, 2008 0196-9781 http://producao.usp.br/handle/BDPI/28612 10.1016/j.peptides.2008.05.001 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER SCIENCE INC |
| Relação |
Peptides |
| Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE INC |
| Palavras-Chave | #spider toxins #Lasiodora #cDNA #expression #calcium channel #venom #PANCREATIC ACINAR-CELLS #EMBRYONAL CARCINOMA-CELLS #MOLECULAR-CLONING #CA2+ RELEASE #VENOM GLAND #1,4,5-TRISPHOSPHATE RECEPTOR #NEURONAL DIFFERENTIATION #INOSITOL TRISPHOSPHATE #RYANODINE RECEPTOR #CA-2+ OSCILLATIONS #Biochemistry & Molecular Biology #Pharmacology & Pharmacy |
| Tipo |
article original article publishedVersion |
