High frequency of immature dendritic cells and altered in situ production of interleukin-4 and tumor necrosis factor-alpha in lung cancer


Autoria(s): BALEEIRO, R. B.; ANSELMO, L. B.; SOARES, F. A.; PINTO, C. A. L.; RAMOS, O.; GROSS, J. L.; HADDAD, F.; YOUNES, R. N.; TOMIYOSHI, M. Y.; BERGAMI-SANTOS, P. C.; BARBUTO, J. A. M.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2008

Resumo

Introduction Antigen-presenting cells, like dendritic cells (DCs) and macrophages, play a significant role in the induction of an immune response and an imbalance in the proportion of macrophages, immature and mature DCs within the tumor could affect significantly the immune response to cancer. DCs and macrophages can differentiate from monocytes, depending on the milieu, where cytokines, like interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) induce DC differentiation and tumor necrosis factor (TNF)-alpha induce DC maturation. Thus, the aim of this work was to analyze by immunohistochemistry the presence of DCs (S100+ or CD1a+), macrophages (CD68+), IL-4 and TNF-alpha within the microenvironment of primary lung carcinomas. Results Higher frequencies of both immature DCs and macrophages were detected in the tumor-affected lung, when compared to the non-affected lung. Also, TNF-alpha-positive cells were more frequent, while IL-4-positive cells were less frequent in neoplastic tissues. This decreased frequency of mature DCs within the tumor was further confirmed by the lower frequency of CD14-CD80+ cells in cell suspensions obtained from the same lung tissues analyzed by flow cytometry. Conclusion These data are discussed and interpreted as the result of an environment that does not oppose monocyte differentiation into DCs, but that could impair DC maturation, thus affecting the induction of effective immune responses against the tumor.

Identificador

CANCER IMMUNOLOGY IMMUNOTHERAPY, v.57, n.9, p.1335-1345, 2008

0340-7004

http://producao.usp.br/handle/BDPI/28611

10.1007/s00262-008-0468-7

http://dx.doi.org/10.1007/s00262-008-0468-7

Idioma(s)

eng

Publicador

SPRINGER

Relação

Cancer Immunology Immunotherapy

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #lung cancer #dendritic cells #macrophages #IL-4 #TNF #immunohistochemistry #CALCIUM-BINDING PROTEINS #LANGERHANS CELLS #S-100 PROTEIN #PROGNOSTIC-SIGNIFICANCE #LYMPHOID ORGANS #S100 FAMILY #CARCINOMA #EXPRESSION #RECEPTORS #ANTIGEN #Oncology #Immunology
Tipo

article

original article

publishedVersion