Tumor-derived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Exposure of phosphatidylserine (PS) on cellular membranes and membrane-derived microvesicles stimulates a number of anti-inflammatory responses involved in malignant processes. Herein we show that B16F10 cells, a highly metastatic melanoma cell line, produce large quantities of PS-containing microvesicles in vitro. Tumor microvesicles increased TGF-beta(1) production by cultured macrophages and, in vivo, enhanced the metastatic potential of B16F10 cells in C57BL/6 mice, both effects being reversed by annexin V. Most strikingly, microvesicles induced melanoma metastasis in BALB/c mice, which are normally resistant to this tumor cell line. Altogether, this is the first demonstration that tumor-derived microvesicles favor the establishment of melanoma metastasis in a PS-dependent manner, possibly by down-regulating the host`s inflammatory and/or anti-tumoral immune responses. (C) 2009 Elsevier Ireland Ltd. All rights reserved. Instituto Nacional do Cancer (INCa)/FundaqAo Ary Frauzino para Pesquisa e Controle do CSncer (FAF) Instituto Nacional do Cancer (INCa)/FundaqAo Ary Frauzino para Pesquisa e Controle do CSncer (FAF) |
Identificador |
CANCER LETTERS, v.283, n.2, p.168-175, 2009 0304-3835 http://producao.usp.br/handle/BDPI/28527 10.1016/j.canlet.2009.03.041 |
Idioma(s) |
eng |
Publicador |
ELSEVIER IRELAND LTD |
Relação |
Cancer Letters |
Direitos |
restrictedAccess Copyright ELSEVIER IRELAND LTD |
Palavras-Chave | #Microvesicles #Melanoma #Metastasis #Phosphatidylserine #TGF-beta(1) #GROWTH-FACTOR-BETA #MEMBRANE PHOSPHOLIPID ASYMMETRY #PLASMA-MEMBRANE #RELEASED MICROVESICLES #MESSENGER-RNA #T-LYMPHOCYTES #TGF-BETA #CELLS #CANCER #VESICLES #Oncology |
Tipo |
article original article publishedVersion |