Comparative phylogeography of Trypanosoma cruzi TCIIc: New hosts, association with terrestrial ecotopes, and spatial clustering


Autoria(s): MARCILI, Arlei; LIMA, Luciana; VALENTE, Vera C.; VALENTE, Sebastiao A.; BATISTA, Jael S.; JUNQUEIRA, Angela C. V.; SOUZA, Alda I.; ROSA, Joao A. da; CAMPANER, Marta; LEWIS, Michael D.; LLEWELLYN, Martin S.; MILES, Michael A.; TEIXEIRA, Marta M. G.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

We characterized 28 new isolates of Trypanosoma cruzi IIc (TCIIc) of mammals and triatomines from Northern to Southern Brazil, confirming the widespread distribution of this lineage. Phylogenetic analyses using cytochrome b and SSU rDNA sequences clearly separated TCIIc from TCIIa according to terrestrial and arboreal ecotopes of their preferential mammalian hosts and vectors. TCIIc was more closely related to TCIId/e, followed by TCIIa, and separated by large distances from TCIIb and TCI. Despite being indistinguishable by traditional genotyping and generally being assigned to Z3, we provide evidence that TCIIa from South America and TCIIa from North America correspond to independent lineages that circulate in distinct hosts and ecological niches. Armadillos, terrestrial didelphids and rodents, and domestic dogs were found infected by TCIIc in Brazil. We believe that, in Brazil, this is the first description of TCIIc from rodents and domestic dogs. Terrestrial triatomines of genera Panstrongylus and Triatoma were confirmed as vectors of TCIIc. Together, habitat, mammalian host and vector association corroborated the link between TCIIc and terrestrial transmission cycles/ecological niches. Analysis of ITS1 rDNA sequences disclosed clusters of TCIIc isolates in accordance with their geographic origin, independent of their host species. (C) 2009 Elsevier B.V. All rights reserved.

CNPq

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

FAPESP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

IEC

IEC

PNPD-CAPES

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

EC[223034]

EC

Identificador

INFECTION GENETICS AND EVOLUTION, v.9, n.6, p.1265-1274, 2009

1567-1348

http://producao.usp.br/handle/BDPI/28515

10.1016/j.meegid.2009.07.003

http://dx.doi.org/10.1016/j.meegid.2009.07.003

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Infection Genetics and Evolution

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Trypanosoma cruzi #Brazil #USA #Chagas disease #Lineages TCIIc and TCIIa #Armadillo #Evolution #Phylogeography #SSU rDNA #Cytochrome b #MULTILOCUS ENZYME ELECTROPHORESIS #RURAL NORTHWESTERN ARGENTINA #AMPLIFIED POLYMORPHIC DNA #CHAGAS-DISEASE #MOLECULAR EPIDEMIOLOGY #PHYLOGENETIC LINEAGES #BRAZILIAN AMAZONIA #SYLVATIC MAMMALS #UNITED-STATES #RIBOSOMAL-RNA #Infectious Diseases
Tipo

article

original article

publishedVersion