In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2010
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Resumo |
Resistance of Leishmania parasites to specific chemotherapy has become a well-documented problem in the Indian subcontinent in recent years but only a few studies have focused on the susceptibility of American Leishmania isolates. Our susceptibility assays to meglumine antimoniate were performed against intracellular amastigotes after standardizing an in vitro model of macrophage infection appropriate for Leishmania (Viannia) braziliensis isolates. For the determination of promastigote susceptibility to amphotericin B, we developed a simplified MTT-test. The sensitivity in vitro to meglumine antimoniate and amphotericin B of 13 isolates obtained from Brazilian patients was determined. L. (V.) braziliensis isolates were more susceptible to meglumine antimoniate than Leishmania (Leishmania) amazonensis. EC(50), EC(90) and activity indexes (calculated over the sensitivity of reference strains), suggested that all isolates tested were susceptible in vitro to meglumine antimoniate, and did not show association with the clinical outcomes. Isolates were also uniformly susceptible in vitro to amphotericin B. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) |
Identificador |
TROPICAL MEDICINE & INTERNATIONAL HEALTH, v.15, n.1, p.68-76, 2010 1360-2276 http://producao.usp.br/handle/BDPI/28510 10.1111/j.1365-3156.2009.02414.x |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL PUBLISHING, INC |
Relação |
Tropical Medicine & International Health |
Direitos |
restrictedAccess Copyright WILEY-BLACKWELL PUBLISHING, INC |
Palavras-Chave | #tegumentary leishmaniasis #meglumine antimoniate #amphotericin B #sensitivity #AMERICAN TEGUMENTARY LEISHMANIASIS #CUTANEOUS LEISHMANIASIS #VISCERAL LEISHMANIASIS #SODIUM STIBOGLUCONATE #TREATMENT FAILURE #DRUG-RESISTANCE #CLINICAL-TRIAL #SUSCEPTIBILITY #PROMASTIGOTES #EPIDEMIOLOGY #Public, Environmental & Occupational Health #Tropical Medicine |
Tipo |
article original article publishedVersion |