In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B


Autoria(s): ZAULI-NASCIMENTO, Rogeria C.; MIGUEL, Danilo C.; YOKOYAMA-YASUNAKA, Jenicer K. U.; PEREIRA, Ledice I. A.; OLIVEIRA, Milton A. Pelli de; RIBEIRO-DIAS, Fatima; DORTA, Miriam L.; ULIANA, Silvia R. B.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

Resistance of Leishmania parasites to specific chemotherapy has become a well-documented problem in the Indian subcontinent in recent years but only a few studies have focused on the susceptibility of American Leishmania isolates. Our susceptibility assays to meglumine antimoniate were performed against intracellular amastigotes after standardizing an in vitro model of macrophage infection appropriate for Leishmania (Viannia) braziliensis isolates. For the determination of promastigote susceptibility to amphotericin B, we developed a simplified MTT-test. The sensitivity in vitro to meglumine antimoniate and amphotericin B of 13 isolates obtained from Brazilian patients was determined. L. (V.) braziliensis isolates were more susceptible to meglumine antimoniate than Leishmania (Leishmania) amazonensis. EC(50), EC(90) and activity indexes (calculated over the sensitivity of reference strains), suggested that all isolates tested were susceptible in vitro to meglumine antimoniate, and did not show association with the clinical outcomes. Isolates were also uniformly susceptible in vitro to amphotericin B.

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificador

TROPICAL MEDICINE & INTERNATIONAL HEALTH, v.15, n.1, p.68-76, 2010

1360-2276

http://producao.usp.br/handle/BDPI/28510

10.1111/j.1365-3156.2009.02414.x

http://dx.doi.org/10.1111/j.1365-3156.2009.02414.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL PUBLISHING, INC

Relação

Tropical Medicine & International Health

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL PUBLISHING, INC

Palavras-Chave #tegumentary leishmaniasis #meglumine antimoniate #amphotericin B #sensitivity #AMERICAN TEGUMENTARY LEISHMANIASIS #CUTANEOUS LEISHMANIASIS #VISCERAL LEISHMANIASIS #SODIUM STIBOGLUCONATE #TREATMENT FAILURE #DRUG-RESISTANCE #CLINICAL-TRIAL #SUSCEPTIBILITY #PROMASTIGOTES #EPIDEMIOLOGY #Public, Environmental & Occupational Health #Tropical Medicine
Tipo

article

original article

publishedVersion